--- title: Technology | PICO BioScience --- [Skip to content](https://www.pico-bioscience.eu/technology-pico-bioscience#main-content) [![PICO BioScience logo](https://www.pico-bioscience.eu/hs-fs/hubfs/Logos/PICO%20and%20actome/PICO%20BioScience%20(no%20background).png?width=1280&height=722&name=PICO%20BioScience%20(no%20background).png)Homepage](https://www.pico-bioscience.eu?hsLang=eu) - Solutions - [Single EV Analysis](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [Protein Detection](https://www.pico-bioscience.eu/protein-detection?hsLang=eu) - [Antibody Labeling](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) - The PICO Workflow - [PICO Technology Overview](https://www.pico-bioscience.eu/technology-pico-bioscience) - [PIQuant Software](https://www.pico-bioscience.eu/piquant?hsLang=eu) - [Instrument Compatibility](https://www.pico-bioscience.eu/instrument-compatibility?hsLang=eu) - [Resources](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Application Notes](https://www.pico-bioscience.eu/application-notes?hsLang=eu) - [Technical Support](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Manuals](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Company](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) - [Our Mission](https://www.pico-bioscience.eu/our-mission?hsLang=eu) - [Careers](https://www.linkedin.com/company/pico-bioscience/) - [Partnerships](https://www.pico-bioscience.eu/collaborative-research?hsLang=eu) - [Contact](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) - Solutions - [Single EV Analysis](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [Protein Detection](https://www.pico-bioscience.eu/protein-detection?hsLang=eu) - [Antibody Labeling](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) - The PICO Workflow - [PICO Technology Overview](https://www.pico-bioscience.eu/technology-pico-bioscience) - [PIQuant Software](https://www.pico-bioscience.eu/piquant?hsLang=eu) - [Instrument Compatibility](https://www.pico-bioscience.eu/instrument-compatibility?hsLang=eu) - [Resources](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Application Notes](https://www.pico-bioscience.eu/application-notes?hsLang=eu) - [Technical Support](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Manuals](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Company](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) - [Our Mission](https://www.pico-bioscience.eu/our-mission?hsLang=eu) - [Careers](https://www.linkedin.com/company/pico-bioscience/) - [Partnerships](https://www.pico-bioscience.eu/collaborative-research?hsLang=eu) - [Contact](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) [Shop](https://pico-bioscience.myshopify.com)[PIQuant](https://pico-bioscience-piquant.share.connect.posit.cloud/) # PICO Technology Overview [![Step1 - Labeling](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-1-Step1-%20Labeling.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-1-Step1-%20Labeling.gif "Step1 - Labeling")](https://landing.actome.de/antibody-labeling) #### **PICO Labeling Service: We Do the Work, You Get the Data** Don't waste time on manual conjugation. Name your targets, and our scientists will handle the entire process from procurement to strict quality control. - **Hands-Off Convenience:** Simply ship your antibodies to our lab, or let us order them directly from your preferred supplier. - **Tailored to Your Assay:** Choose the exact labels your specific multiplex assay requires. - **100% Site-Directed Precision:** We guarantee flawless, reproducible coupling targeting non-variable regions—leaving your antigen-binding sites completely untouched. - **Exact Stoichiometry:** Our standardized process ensures absolute molecular consistency, loading exactly 2 labels per heavy chain. - **Built-In Quality Control:** Every batch undergoes rigorous testing to verify the final concentration and absolute labeling efficiency. **Requirement:** This premium service is optimized for **IgG antibodies**. #### **The PICO Binding Reaction** No complex washing steps, no surface immobilization. Just mix your purified sample with the labeled antibodies and let the molecular proximity do the rest. - **Solution-Based Binding:** The entire reaction happens completely in solution. Your targets maintain their native conformation, ensuring realistic, unhindered biological interactions. - **No Surface Loss:** Because there are no washing steps or solid-phase binding, you completely eliminate the background noise and sample loss typical of traditional assays. - **Intact Membrane Integrity:** For EV analysis, the entire binding reaction is completely detergent-free. Your vesicles remain 100% intact from the initial mix to the final readout. ![Step2 - Binding](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-2-Step2-%20Binding%201.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-2-Step2-%20Binding%201.gif "Step2 - Binding") ![Step3 - Amplification](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-3-Step3-%20Amplification.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-3-Step3-%20Amplification.gif "Step3 - Amplification") #### **Digital PCR Partitioning & Multiplex Amplification** This is where the biochemistry turns into digital data. The sample mixture is loaded directly into the digital PCR system, whether it utilizes microfluidic nanoplates, chips, or droplet generation. - **Micro-Partitioning:** The sample is instantly distributed across thousands of isolated nanoliter cavities or oil droplets. Individual targets and their bound DNA-labeled antibodies are locked into separate, independent micro-reactions. - **Proximity-Driven Amplification:** Once partitioned, the standard dPCR master mix amplifies the DNA barcodes. A distinct fluorescent signal is generated *only* if the specific target markers are present together in that individual partition. - **Multiplexing Opportunities:** By using the multiple fluorescent dye channels on your dPCR hardware, you can simultaneously detect different target combinations, subpopulation markers, or distinct protein complexes in a single run. - **Direct Molecular Counting:** Because dPCR operates on a binary (positive/negative) readout per partition, the system counts the absolute number of fluorescent events. No reference standards or standard curves required. ### **Automated Data Analysis with PIQuant® Software** Once your digital PCR run is complete, the raw data is seamlessly converted into clear, actionable results without manual calculation or complex spreadsheets. - **Effortless Upload:** Simply upload the raw data files directly from your dPCR instrument into the intuitive PIQuant® software interface. - **Automated Partition Counting:** PIQuant® instantly scans and analyzes each cavity, compartment, or droplet, identifying the exact ratio of positive-to-negative fluorescent signals. - **Patented Quantification Algorithm:** The software utilizes a unique, patented algorithm to filter and calculate the absolute molecular concentration of your target complexes. - **Zero Calibration Needed:** Because the algorithm provides direct, absolute quantification, you get precise molecular counts without ever needing to run a standard reference curve. ![Step4 - Analysis](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-4-Step4-%20Analysis.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-4-Step4-%20Analysis.gif "Step4 - Analysis") - ![Unbenannt](https://www.pico-bioscience.eu/hs-fs/hubfs/Unbenannt.png?width=1080&height=1080&name=Unbenannt.png) **EV Quantification and Characterization** - ![PICO\_single\_protein](https://www.pico-bioscience.eu/hs-fs/hubfs/PICO_single_protein.png?width=1080&height=1080&name=PICO_single_protein.png) **Protein Detection** - [![PICO\_Labeling\_Service](https://www.pico-bioscience.eu/hs-fs/hubfs/PICO_Labeling_Service.png?width=1080&height=1080&name=PICO_Labeling_Service.png) **Labeling Service**](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) ## Next-Gen EV Analysis: Beyond the Limits of Size Using **PICO** shifts the focus from physical particle size to molecular surface markers, allowing you to see what traditional optical methods miss. - **Size-Independent Quantification:** Detect and count small vesicles (\<100 nm) just as reliably as larger ones—bypassing the physical limits of NTA and flow cytometry. - **Subpopulation Profiling:** Identify and isolate specific vesicle populations based on surface marker composition (e.g., tumor-specific markers like HER2+). - **True Co-Occurrence Analysis:** Prove that multiple markers are physically present on the *same individual* vesicle membrane, rather than just co-existing in bulk solution. - **1 µL Sample Conservation:** Achieve 100x higher sensitivity than ELISA, extracting maximum digital data from precious, low-volume purified samples. **Stop guessing by size. Start counting by biology. PICO makes it count!** ## Protein, Protein-Protein interaction detection The PICO technology redefines how you detect and measure protein-protein interactions (PPIs) and low-abundance proteins, replacing messy bulk assays with digital precision. - **True Dimer & Complex Detection:** Quantify physical protein-protein interactions directly in solution, proving two targets are bound as a complex rather than just co-existing. - **200x Higher Sensitivity than Co-IP:** Capture transient or weak interactions and ultra-low abundance proteins that are completely invisible to Western Blots or traditional Co-IP. - **No Surface Matrix Bias:** The entire assay takes place completely in solution. Eliminate the background noise, washing artifacts, and sample loss caused by solid-phase binding. - **Ultra-Low Sample Input:** Map complex protein networks using just a fraction of a single cell lysate microliter, conserving precious clinical materials. **Stop masking interactions. Start counting complexes. PICO makes it count!** ## Site-Specific Antibody Labeling Service Our premium **PICO Custom Labeling Service** handles the entire conjugation process for you, turning your standard IgG antibodies into high-performance digital detection units. - **Hands-Off Convenience:** Simply ship your antibodies to our lab, or let us order them directly from your preferred supplier. - **100% Site-Directed Precision:** We guarantee reproducible coupling targeting only non-variable regions, leaving your critical antigen-binding sites completely untouched. - **Exact Stoichiometry:** Our standardized molecular process ensures absolute consistency, loading exactly 2 DNA labels per heavy chain. - **Guaranteed Quality Control:** Every batch undergoes rigorous testing to verify the precise final concentration and absolute labeling efficiency before it returns to your lab. **You ship the binders, we deliver digital precision. PICO makes it count!** **[ Privacy Policy ·](https://www.pico-bioscience.eu/privacy-policy?hsLang=eu) [General Terms and Conditions](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) · [Imprint/Impressum](https://www.pico-bioscience.eu/imprint?hsLang=eu)**© 2026 **·** All rights reserved