--- title: Digital Proteomics | Absolute Quantification of PTMs and PPIs | PICO description: Quantify single proteins, PPIs, and PTMs in a single dPCR run. Bypass bulk ensemble averaging with our quadratic assay design. PICO makes it count! --- [Skip to content](https://www.pico-bioscience.eu/protein-detection#main-content) [![PICO BioScience logo](https://www.pico-bioscience.eu/hs-fs/hubfs/Logos/PICO%20and%20actome/PICO%20BioScience%20(no%20background).png?width=1280&height=722&name=PICO%20BioScience%20(no%20background).png)Homepage](https://www.pico-bioscience.eu?hsLang=eu) - Solutions - [Single EV Analysis](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [Protein Detection](https://www.pico-bioscience.eu/protein-detection) - [Antibody Labeling](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) - The PICO Workflow - [PICO Technology Overview](https://www.pico-bioscience.eu/technology-pico-bioscience?hsLang=eu) - [PIQuant Software](https://www.pico-bioscience.eu/piquant?hsLang=eu) - [Instrument Compatibility](https://www.pico-bioscience.eu/instrument-compatibility?hsLang=eu) - [Resources](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Application Notes](https://www.pico-bioscience.eu/application-notes?hsLang=eu) - [Technical Support](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Manuals](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Company](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) - [Our Mission](https://www.pico-bioscience.eu/our-mission?hsLang=eu) - [Careers](https://www.linkedin.com/company/pico-bioscience/) - [Partnerships](https://www.pico-bioscience.eu/collaborative-research?hsLang=eu) - [Contact](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) - Solutions - [Single EV Analysis](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [Protein Detection](https://www.pico-bioscience.eu/protein-detection) - [Antibody Labeling](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) - The PICO Workflow - [PICO Technology Overview](https://www.pico-bioscience.eu/technology-pico-bioscience?hsLang=eu) - [PIQuant Software](https://www.pico-bioscience.eu/piquant?hsLang=eu) - [Instrument Compatibility](https://www.pico-bioscience.eu/instrument-compatibility?hsLang=eu) - [Resources](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Application Notes](https://www.pico-bioscience.eu/application-notes?hsLang=eu) - [Technical Support](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Manuals](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Company](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) - [Our Mission](https://www.pico-bioscience.eu/our-mission?hsLang=eu) - [Careers](https://www.linkedin.com/company/pico-bioscience/) - [Partnerships](https://www.pico-bioscience.eu/collaborative-research?hsLang=eu) - [Contact](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) [Shop](https://pico-bioscience.myshopify.com)[PIQuant](https://pico-bioscience-piquant.share.connect.posit.cloud/) ### **Digital Proteomics: Single Proteins, Interactions & PTMs** Analyze complex protein–protein interactions (PPIs) and post-translational modifications (PTMs) simultaneously in a single, streamlined assay. By eliminating the limitations of traditional bulk ensemble averaging, PICO delivers absolute quantitative clarity at the molecular level. ### **Target Your Biomarkers with Dual-Antibody Precision** Using a highly specific dual-antibody proximity mechanism, PICO allows you to precisely target, characterize, and count your specific molecules of interest. Maximize your insights across three core applications: - **Single Proteins:** Direct absolute counting of low-abundance targets without reference standards. - **Post-Translational Modifications (PTMs):** Quantify specific phosphorylation, methylation, or activation states directly on the target protein. - **Protein-Protein Interactions (PPIs):** Detect and verify physical dimer formation and multi-protein complexes in native solution. ![PICO\_Targets-animated](https://www.pico-bioscience.eu/hs-fs/hubfs/PICO_Targets-animated.gif?width=2000&height=2000&name=PICO_Targets-animated.gif "PICO_Targets-animated") ### Absolute Quantification ### Multiplex Analysis ### High Throughput ### Specific and Reproducible ### Reference and Standard free Overcoming coIP Limitations Traditional PPI studies like Co-IP demand 1–3 mg of total protein, often forcing researchers into artificial cell overexpression. PICO shatters this input barrier. Capture robust, reproducible data on transient interactions and ultra-low abundance targets directly from minute, native sample volumes—completely eliminating the risk of overexpression artifacts.  Quantifying Drug Effects Track cellular responses and molecular pathway dynamics with ultimate sensitivity. PICO precisely quantifies subtle perturbations in signaling pathways—such as tracking the induction or inhibition of ERBB2:ERBB3 dimeric interactions following targeted drug treatments (e.g., Dactolisib). Get clinically relevant, high-resolution data when it matters most. Simultaneous Detection Why waste time and sample running multiple individual assays? Our innovative assay design allows for the simultaneous quantification of the total protein amounts for *both* individual targets alongside their specific physical interaction,  all in a single, automated digital PCR run. Request a Technical CallPPI Application Note **[ Privacy Policy ·](https://www.pico-bioscience.eu/privacy-policy?hsLang=eu) [General Terms and Conditions](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) · [Imprint/Impressum](https://www.pico-bioscience.eu/imprint?hsLang=eu)**© 2026 **·** All rights reserved